Mapping Short Affinity Tags on Bacterial S-layer with Antibody
Sprache des Titels:
In conclusion, the interaction between the Strep-tagII and anti-Strep-tagII was explored at the single molecular level. Strep-tagII in the two-dimensional crystalline S-layer protein lattices on a silicon surface could be detected by anti-Strep-tagII antibodies coupled to AFM tip using single-molecule force spectroscopy. The unbinding force between Strep-tagII peptide and anti-Strep-tagII antibody was 27 pN at a loading rate of 177 pNs1.