Markus Axmann, J. B. Huppa, Mark M. Davis, Gerhard Schütz,
"Determination of Interaction Kinetics between the T Cell Receptor and Peptide-Loaded MHC Class II via Single-Molecule Diffusion Measurements"
, in Biophysical Journal, Vol. 103, Nummer 2, Seite(n) L17-L19, 7-2012, ISSN: 1542-0086
Determination of Interaction Kinetics between the T Cell Receptor and Peptide-Loaded MHC Class II via Single-Molecule Diffusion Measurements
Sprache des Titels:
The binding of peptide-loaded major histocompatibility complex (pMHC) to the T cell receptor (TCR) represents
the central step in T cell antigen recognition. It proceeds in the cell contact area between a T cell and an antigen-presenting cell
termed the immunological synapse. An important and unresolved issue is how T cells discriminate between potentially harmful
and harmless antigens. One limitation has been the difficulty to measure interaction parameters directly, that is, as they
occur in the immunological synapse. Here we present a single-molecule approach to determine pMHC-TCR interaction kinetics
in situ based on diffusion analysis of dye-labeled pMHC. We find synaptic off-rates >10-fold accelerated when compared to the
dissociation of purified proteins measured in vitro.