Werner Baumgartner, Nikola Golenhofen, Niko Grundhöfer, Johannes Wiegand, Detlev Drenckhahn,
"Ca2+ dependency of N-cadherin function probed by laser tweezer and atomic force microscopy"
, in The Journal of Neuroscience: The Official Journal of the Society for Neuroscience, Vol. 23, Nummer 35, Seite(n) 11008--11014, 2003, ISSN: 1529-2401
Original Titel:
Ca2+ dependency of N-cadherin function probed by laser tweezer and atomic force microscopy
Sprache des Titels:
Englisch
Original Kurzfassung:
This study was undertaken to provide a biophysical basis for the hypothesis that activity-dependent modulation of cadherin-mediated adhesion by transient changes of extracellular calcium ([Ca2+]e) is causally involved in coordination of synaptic plasticity. Characterization of homophilic N-cadherin binding by atomic force microscopy and laser tweezer trapping of N-cadherin-coated microbeads attached to the cell surface of cultured neuronal cells showed that adhesive activity of N-cadherin is effectively regulated between 0.3 and 0.8 mm [Ca2+]e. Furthermore, we show that an increase of [Ca2+]i, which is known to be essential for induction of synaptic plasticity, causes significant reduction of cadherin-mediated bead adhesion that could be completely suppressed by inhibition of actin depolymerization. The results of this study show that N-cadherin has ideal biophysical properties to serve as a Ca2+-dependent sensor for synaptic activity and, at the same time, is strategically located to control synaptic adhesion. A drop of [Ca2+]e and a concomitant increase of [Ca2+]i may act in concert to modulate N-cadherin-based adhesive contacts at synaptic sites.
Sprache der Kurzfassung:
Englisch
Journal:
The Journal of Neuroscience: The Official Journal of the Society for Neuroscience
Forschungs-