Covalent attachment of proteins to calcite surface for the single molecule experiments
Sprache des Titels:
Two protocols of covalent attachment of proteins onto the calcite surface, viz. one using the metallochelat and second using the aminohexil,
are elaborated. Single molecule force spectroscopy method has been used to test their efficiency and practical applicability. Experiments were
performed measuring the specific interaction force between bovine serum albumin (BSA) fixed onto the freshly cleaved calcite single crystal
surface (procedure under the study here) and its polyclonal antibody (Ab-BSA) immobilized onto an AFM tip using standard and well studied
procedure. We found the conditions, when up to 3?3.5% of tip-sample approaches lead to the formation of a single specific bond.