Characterization of extrinsic protein PsbP from Photosystem II by solution NMR spectroscopy
Sprache des Vortragstitels:
Englisch
Original Tagungtitel:
BISSL (Bilateral Impromptu Symposium (Stockholm-Linz) on NMR Spectroscopy
Sprache des Tagungstitel:
Englisch
Original Kurzfassung:
Psb proteins of photosystem II (PSII) are a class of extrinsic proteins located in
the cytosolic part of the oxygen evolving complex (OEC). They help to maintain
proper conditions for the water splitting reaction ultimately leading to the release of
molecular oxygen1. Each of these proteins (PsbP, PsbQ, PsbO) contains function-
ally indispensable intrinsically disordered regions (IDRs), which are not resolved in
X-ray diffraction data. Solution NMR spectroscopy therefore is the preferred ap-
proach to assess their structural propensities, dynamics and interactions. We have
determined three-dimensional structures of PsbP (23 kDa) and PsbQ (16 kDa) in
solution including their highly flexible IDRs, which were incompletely characterized
by other structural biology techniques (X-ray, cryo-electron microscopy)2-6. The
dynamic interactions of the Psb proteins have been studied using combination of
NMR techniques such as chemical shift perturbation (CSP) titration and chemical
exchange saturation transfer (CEST) as well other methods like isothermal titration
calorimetry (ITC) and microscale thermophoresis (MST).
Forschungs-