Parallel DNA Amplification Using Localized Microwave Heating in Standard Microtubes
Sprache des Vortragstitels:
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS)
Sprache des Tagungstitel:
This work presents the utilization of an open-ended semirigid coaxial cable as a miniaturized microwave heating source used in standard 0.2ml microtubes for PCR amplification of single DNA-molecules. With our set-up we achieved the successful amplification of single molecules in a microscopic format. Specifically, single molecules are compartmentalized in microscopic reaction volumes formed by an emulsion. The amplification product is captured on microscopic paramagnetic beads that can then be analyzed under a microscope. Using this small PCR format, we have proof of principle that our device can be used in miniature PCR and is suitable for lab-on-a-chip developments. Moreover, the ease of fabrication and the utilization of standard power RF-components make this set-up suitable for mobile developments also in other technologies.